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- Title
Yellow Fever Virus Genotyping Tool and Investigation of Suspected Adverse Events Following Yellow Fever Vaccination.
- Authors
Rezende, Izabela Maurício de; Alves, Pedro Augusto; Arruda, Matheus Soares; Gonçalves, Andreza Parreiras; Oliveira, Gabriela Fernanda Garcia; Pereira, Leonardo Soares; Dutra, Maria Rita Teixeira; Campi-Azevedo, Ana Carolina; Valim, Valéria; Tourinho, Renata; Oliveira, Jaquelline Germano de; Calzavara, Carlos Eduardo; Said, Rodrigo Fabiano do Carmo; Kroon, Erna Geessien; Martins-Filho, Olindo Assis; Teixeira-Carvalho, Andrea; Drumond, Betânia Paiva
- Abstract
The yellow fever (YF) vaccine consists of an attenuated virus, and despite its relative safety, some adverse events following YF vaccination have been described. At the end of 2016, Brazil experienced the most massive sylvatic yellow fever outbreak over the last 70 years and an intense campaign of YF vaccination occurred in Minas Gerais state in Southeast Brazil from 2016 to 2018. The present study aimed to develop a genotyping tool and investigate 21 cases of suspected adverse events following YF vaccination. Initial in silico analyses were performed using partial NS5 nucleotide sequences to verify the discriminatory potential between wild-type and vaccine viruses. Samples from patients were screened for the presence of the YFV RNA, using 5′UTR as the target, and then used for amplification of partial NS5 gene amplification, sequencing, and phylogenetic analysis. Genotyping indicated that 17 suspected cases were infected by the wild-type yellow fever virus, but four cases remained inconclusive. The genotyping tool was efficient in distinguishing the vaccine from wild-type virus, and it has the potential to be used for the differentiation of all yellow fever virus genotypes.
- Subjects
MINAS Gerais (Brazil); BRAZIL; YELLOW fever; PHYTOPLASMAS; VACCINATION; ADVERSE health care events; NUCLEOTIDE sequence
- Publication
Vaccines, 2019, Vol 7, Issue 4, p206
- ISSN
2076-393X
- Publication type
Article
- DOI
10.3390/vaccines7040206