We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A High-Throughput Screening Procedure (Py-Fe3+) for Enhancing Ethanol Production by Saccharomyces cerevisiae Using ARTP Random Mutagenesis.
- Authors
Wang, Wei-Yang; Wu, Shi-Hua; Xie, Yuan-Han; Zhong, Miao; Wei, Man-Li; Li, Ze-Yang; Long, Xiu-Feng; Niu, Fu-Xing
- Abstract
Saccharomyces cerevisiae is an important microbial organization involved in ethanol synthesis. Mutant strains that can withstand multiple pressures during this process are critical to the industrial development of biofuels. In this study, a dual high-throughput screening method of Triphenyl-2H-tetrazoliumchloride (TTC)-based macroscopic observation and the reaction of ferric nitrate with pyruvate (or pyruvate radical ion) in fermentation broth was used. Using this, an S. cerevisiae mutant library that could tolerate 381 g/L sucrose was established by ARTP random mutation and adaptive evolution to select the best strain; its ethanol yield was increased by an additional 20.48%; and the sucrose utilization rate was 81.64%. This method is specific to the selection of strains with increased ethanol production.
- Subjects
HIGH throughput screening (Drug development); SACCHAROMYCES cerevisiae; FERRIC nitrate; MUTAGENESIS; RADICAL ions; ETHANOL; SUCROSE
- Publication
Processes, 2022, Vol 10, Issue 11, p2186
- ISSN
2227-9717
- Publication type
Article
- DOI
10.3390/pr10112186