We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Semen quality and frozen semen production in Pasundan bulls: A molecular weight perspective on seminal plasma and spermatozoa protein.
- Authors
Baharun, Abdullah; Rahmi, Annisa; Handarini, Ristika; Maulana, Tulus; Said, Syahruddin; Iskandar, Hikmayani; Darussalam, Imam; Nalley, Wilmientje Marlene Mesang; Arifiantini, Raden Iis
- Abstract
Objective: To determine the correlation between the molecular weight (MW) of proteins in seminal plasma and spermatozoa and the quality of fresh and frozen semen production in Pasundan bulls. Materials and Methods: Nine selected Pasundan bulls, aged 5-10 years, from the Regional Artificial Insemination Center at Ciamis, West Java, Indonesia, were used in the study, with fresh semen sperm motility =70% and <70%. We analyzed the motility, viability, integrity of the intact plasma membrane (IPM), and the morphological characteristics of spermatozoa. 1D-SDS-PAGE analysis was performed to determine the protein profile by assessing MW, depicted as bands on the gel. Results: The motility, viability, and IPM of spermatozoa had lower values (p < 0.05) in Pasundan bulls named Bagaskara and Kertarajasa compared to the other bulls. Proteins with MW 35-50 kDa were not detected in the seminal plasma of Pasundan bulls, exhibiting low quality in fresh semen. The correlation analysis showed that the non-detected proteins with MW 35-50 kDa in seminal plasma correlated with spermatozoa motility (r = 0.421), viability (r = 0.424), and IPM (r = 0.428) so that fresh semen quality was low in both Pasundan bulls. Analysis of semen volume, spermatozoa concentration, and spermatozoa motility showed that the average frozen semen production of Pasundan bulls per ejaculate was 128.73 ± 15.35 straws. Conclusion: Protein analysis based on MW is a predictive indicator for the quality of fresh semen and the production of frozen semen in Pasundan bulls. Evaluation parameters of fresh semen quality by MW analysis can be used to select Pasundan bulls in Indonesia.
- Subjects
INDONESIA; JAWA Barat (Indonesia); FROZEN semen; SEMINAL proteins; SEMEN analysis; MOLECULAR weights; SPERM motility; BULLS
- Publication
Journal of Advanced Veterinary & Animal Research, 2023, Vol 10, Issue 4, p730
- ISSN
2311-7710
- Publication type
Article
- DOI
10.5455/javar.2023.j728