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- Title
槲皮素对乳腺癌 MCF-7 和 MDA-MB-435 细胞的凋亡作用及机制研究.
- Authors
甘霖; 熊志峰; 杨化超; 王鸿林; 王婷; 吕钢
- Abstract
Objective: To explore the apoptosis effect of quercetin on the breast cancer MCF-7 and MDA-MB-435 cells, and to explore its mechanism. Methods: The cell counting kit-8 (CCK 8) method was used to determine the effect of quercetin on proliferation of breast cancer MCF-7 and MDA-MB-435 cells, the cell scratch test and the Transwell test were respectively used to determine the effect of quercetin on migration and invasion of breast cancer MCF-7 and MDA-MB-435 cells. Flow cytometry was used to determine the effect of quercetin on apoptosis in breast cancer MCF-7 and MDA-MB-435 cells. The real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) and West-blotting (West-blotting) were used to determine the effect of quercetin on mRNA and protein expression of Fas, FasL, Bcl-2 and Bax in breast cancer MCF-7 and MDA-MB-435 cells. Results: Quercetin (50~200 μmol/L) significantly inhibited the proliferation of breast cancer MCF-7 and MDA-MB-435 cells for 24 h, 48 h and 72 h, and it was concentration dependent manner (P<0.05). The cell scratch width of breast cancer MCF-7 and MDA-MB-435 cells by quercetin 50 μmol/L and 100 μmol/L were significantly increased in cell scratch test compared with those in control group (P<0.05). In Transwell test, the penetrating cells of breast cancer MCF-7 and MDA-MB-435 by quercetin 50 μmol/L and 100 μmol/L were significantly reduced compared with those in the control group (P<0.05). Compared with those in the control group, the cell apoptosis rate of breast cancer MCF-7 and MDA-MB-435 by quercetin 50 μmol/L and 100 μmol/L were significantly increased(P<0.05). Compared with those in the control group, the expression of Bcl-2 mRNA of breast cancer MCF-7 and MDA-MB-435 cells by quercetin 50 μmol/L and 100 μmol/L were significantly decreased (P<0.05), while the expressions of Fas, FasL and Bax mRNA were significantly increased compared with those in the control group (P<0.05). The protein expression of Bcl-2 of breast cancer MCF-7 and MDA-MB-435 cells by quercetin 50 μmol/L and 100 μmol/L were significantly reduced compared with those in the control group (P<0.05), while the protein expression of Fas, FasL and Bax were significantly increased compared with those in the control group (P<0.05). Conclusion: Quercetin can promote the apoptosis of breast cancer cells, which may be related to the activation of exogenous apoptosis pathway by acting on the Fas/FasL apoptosis pathway, and the activation of endogenous apoptosis pathway by acting on the Bcl-2 apoptosis pathway.
- Subjects
FAS proteins; POLYMERASE chain reaction; BREAST cancer; QUERCETIN; PROTEIN expression
- Publication
Progress in Modern Biomedicine, 2021, Vol 21, Issue 19, p3638
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2021.19.008