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- Title
In Vitro Neuroprotective Effects of Centella asiatica.
- Authors
Ismail, Norhayati; Osman, Asiah; Yunos, Nurhanan Murni; Montar, Mastura; Jauri, Muhammad Haffiz
- Abstract
Introduction: The generation of free radicals and oxidative stress has been linked to several neurodegenerative diseases including Parkinson's disease and Alzheimer's disease. The determination of free radical scavenging agents for the reduction of intracellular reactive oxygen species is one of the approaches used in the clinical management of neurodegeneration. Centella asiatica is an important medicinal plant with an extensive range of ethnomedicinal uses that have been reported to exhibit ranges of bioactivity. Methods: Bioassay-guided fractionation was performed in this study to determine the active fraction of C. asiatica which responds to neuroprotective effects on H2O2-induced neurotoxic neuroblastoma SH-SY5Y cell lines. The results were expressed as a percentage of cell viability which was determined using an MTT assay after each of the experiments. Results: In comparison to ethanolic extract, post-treatment with methanolic extracts at 0.49-125 g/ml demonstrated the best protection against H2O2-induced cells by enhancing cell viability. Six fractions were produced by further bioassay-guided separation of the methanolic extract. All fractions exhibit neuroprotective effects at concentrations ranging from 0.49 to 1 25 g/ml, which enhanced the vitality of H2O2-induced SH-SY5Y cells. Conclusion: The treatment of C. asiatica on H2O2-induced neurotoxicity showed promise, according to the findings. Taken together, this study may suggest C. asiatica as a potential therapeutic treatment for H2O2-induced neurotoxicity and further study should be done to investigate the active compound attributed to these neuroprotective effects.
- Subjects
CENTELLA asiatica; ALZHEIMER'S disease; PARKINSON'S disease; REACTIVE oxygen species; NEUROPROTECTIVE agents
- Publication
Malaysian Journal of Medicine & Health Sciences, 2024, Vol 20, p91
- ISSN
1675-8544
- Publication type
Abstract