We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A group of related cDNAs encoding secreted proteins from Hessian fly [ Mayetiola destructor (Say)] salivary glands.
- Authors
Chen, M. -S.; Fellers, J. P.; Stuart, J. J.; Reese, J. C.; Liu, X.
- Abstract
A group of cDNAs has been isolated and characterized from Hessian fly [ Mayetiola destructor (Say)] salivary glands. Members in this group appear to encode proteins with secretion signal peptides at the N-terminals. The mature putative proteins are small, basic proteins with calculated molecular weights that ranged from 8.5 to 10 kDa, and isoelectric points from 9.92 to 10.90. Sequence analysis indicated a strong selection for mutations that generate amino acid changes within the coding region. Northern blot analysis revealed that these genes are expressed only in the first instar larvae, a critical stage that determines if the interaction between a specific Hessian fly biotype and a specific wheat cultivar is compatible. Genomic analysis demonstrated that multiple copies of similar genes are clustered within a short region on chromosome 2A. This is the same arm in which two avirulence genes have been mapped.
- Subjects
HESSIAN fly; DNA; SALIVARY glands; PROTEINS; SECRETION; MAYETIOLA; GENES
- Publication
Insect Molecular Biology, 2004, Vol 13, Issue 1, p101
- ISSN
0962-1075
- Publication type
Article
- DOI
10.1111/j.1365-2583.2004.00465.x