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- Title
The Annexin A2/p11 complex is required for efficient invasion of Salmonella Typhimurium in epithelial cells.
- Authors
Jolly, Carrie; Winfree, Seth; Hansen, Bryan; Steele‐Mortimer, Olivia
- Abstract
The facultative intracellular pathogen, Salmonella enterica, triggers its own uptake into non-phagocytic epithelial cells. Invasion is dependent on a type 3 secretion system ( T3 SS), which delivers a cohort of effector proteins across the plasma membrane where they induce dynamic actin-driven ruffling of the membrane and ultimately, internalization of the bacteria into a modified phagosome. In eukaryotic cells, the calcium- and phospholipid-binding protein Annexin A2 ( AnxA2) functions as a platform for actin remodelling in the vicinity of dynamic cellular membranes. AnxA2 is mostly found in a stable heterotetramer, with p11, which can interact with other proteins such as the giant phosphoprotein AHNAK. We show here that AnxA2, p11 and AHNAK are required for T3 SS-mediated Salmonella invasion of cultured epithelial cells and that the T3 SS effector SopB is required for recruitment of AnxA2 and AHNAK to Salmonella invasion sites. Altogether this work shows that, in addition to targeting Rho-family GTPases, Salmonella can intersect the host cell actin pathway via AnxA2.
- Subjects
ANNEXINS; SALMONELLA typhimurium; EPITHELIAL cells; PATHOGENIC microorganisms; SALMONELLA enterica; CELL membranes; ACTIN; PHAGOSOMES
- Publication
Cellular Microbiology, 2014, Vol 16, Issue 1, p64
- ISSN
1462-5814
- Publication type
Article
- DOI
10.1111/cmi.12180