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- Title
Poly (A)-specific ribonuclease deficiency impacts oogenesis in zebrafish.
- Authors
Nanjappa, Dechamma Pandyanda; De Saffel, Hanna; Kalladka, Krithika; Arjuna, Srividya; Babu, Nishith; Prasad, Kishan; Sips, Patrick; Chakraborty, Anirban
- Abstract
Poly (A)-specific ribonuclease (PARN) is the most important 3′–5′exonuclease involved in the process of deadenylation, the removal of poly (A) tails of mRNAs. Although PARN is primarily known for its role in mRNA stability, recent studies suggest several other functions of PARN including a role in telomere biology, non-coding RNA maturation, trimming of miRNAs, ribosome biogenesis and TP53 function. Moreover, PARN expression is de-regulated in many cancers, including solid tumours and hematopoietic malignancies. To better understand the in vivo role of PARN, we used a zebrafish model to study the physiological consequences of Parn loss-of-function. Exon 19 of the gene, which partially codes for the RNA binding domain of the protein, was targeted for CRISPR-Cas9-directed genome editing. Contrary to the expectations, no developmental defects were observed in the zebrafish with a parn nonsense mutation. Intriguingly, the parn null mutants were viable and fertile, but turned out to only develop into males. Histological analysis of the gonads in the mutants and their wild type siblings revealed a defective maturation of gonadal cells in the parn null mutants. The results of this study highlight yet another emerging function of Parn, i.e., its role in oogenesis.
- Subjects
RIBONUCLEASES; RNA-binding proteins; OOGENESIS; BRACHYDANIO; ORGANELLE formation; EXONUCLEASES; TELOMERASE; MESSENGER RNA
- Publication
Scientific Reports, 2023, Vol 13, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-023-37226-6