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- Title
Engineering a Microphysiological Model for Regenerative Endodontic Studies.
- Authors
Sanz-Serrano, Diana; Mercade, Montse; Ventura, Francesc; Sánchez-de-Diego, Cristina
- Abstract
Simple Summary: Dental pulp infections are common oral health problems that require thorough treatment to disinfect and prepare the root canal using irrigating solutions. However, research on regenerative procedures in endodontics, particularly those involving immature root canals, has been hindered by the lack of suitable laboratory models. In response, this study aimed to create a 3D microphysiological system (MPS) to mimic immature root canals and test the effects of different irrigating solutions. By using human stem cell-derived DSCS cells, researchers found that some irrigating solutions reduced cell viability and affected cell adhesion in the MPS. Notably, this study identified two irrigating solutions, 17% EDTA and 9% HEBP, that showed promising results in terms of cell viability and adherence in the 3D MPS model. These findings emphasize the importance of the MPS for studying root canal treatments and suggest potential alternatives to traditional irrigating solutions for clinical use. This research could lead to improved treatments for dental pulp infections, benefiting patients and dental practitioners alike. Dental pulp infections are common buccal diseases. When this happens, endodontic treatments are needed to disinfect and prepare the root canal for subsequent procedures. However, the lack of suitable in vitro models representing the anatomy of an immature root canal hinders research on regenerative events crucial in endodontics, such as regenerative procedures. This study aimed to develop a 3D microphysiological system (MPS) to mimic an immature root canal and assess the cytotoxicity of various irrigating solutions on stem cells. Utilizing the Dental Stem Cells SV40 (DSCS) cell line derived from human apical papilla stem cells, we analyzed the effects of different irrigants, including etidronic acid. The results indicated that irrigating solutions diminished cell viability in 2D cultures and influenced cell adhesion within the microphysiological device. Notably, in our 3D studies in the MPS, 17% EDTA and 9% 1-hydroxyethylidene-1, 1-bisphosphonate (HEBP) irrigating solutions demonstrated superior outcomes in terms of DSCS viability and adherence compared to the control. This study highlights the utility of the developed MPS for translational studies in root canal treatments and suggests comparable efficacy between 9% HEBP and 17% EDTA irrigating solutions, offering potential alternatives for clinical applications.
- Subjects
ENGINEERING models; ROOT canal treatment; DENTAL pulp; MICROPHYSIOLOGICAL systems; REGENERATION (Biology); CELL adhesion
- Publication
Biology (2079-7737), 2024, Vol 13, Issue 4, p221
- ISSN
2079-7737
- Publication type
Article
- DOI
10.3390/biology13040221