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- Title
Ubiquitin binding mediates the NF-κB inhibitory potential of ABIN proteins.
- Authors
Wagner, S.; Carpentier, I.; Rogov, V.; Kreike, M.; Ikeda, F.; Löhr, F.; Wu, C.-J.; Ashwell, J. D.; Dötsch, V.; Dikic, I.; Beyaert, R.
- Abstract
Deregulated nuclear factor κB (NF-κB) activation plays an important role in inflammation and tumorigenesis. ABIN proteins have been characterized as negative regulators of NF-κB signaling. However, their mechanism of NF-κB inhibition remained unclear. With the help of a yeast two-hybrid screen, we identified ABIN proteins as novel ubiquitin-interacting proteins. The minimal ubiquitin-binding domain (UBD) corresponds to the ABIN homology domain 2 (AHD2) and is highly conserved in ABIN-1, ABIN-2 and ABIN-3. Moreover, this region is also present in NF-κB essential modulator/IκB kinase γ (NEMO/IKKγ) and the NEMO-like protein optineurin, and is therefore termed UBD in ABIN proteins and NEMO (UBAN). Nuclear magnetic resonance studies of the UBAN domain identify it as a novel type of UBD, with the binding surface on ubiquitin being significantly different from the binding surface of other UBDs. ABIN-1 specifically binds ubiquitinated NEMO via a bipartite interaction involving its UBAN and NEMO-binding domain. Mutations in the UBAN domain led to a loss of ubiquitin binding and impaired the NF-κB inhibitory potential of ABINs. Taken together, these data illustrate an important role for ubiquitin binding in the negative regulation of NF-κB signaling by ABINs and identify UBAN as a novel UBD.Oncogene (2008) 27, 3739–3745; doi:10.1038/sj.onc.1211042; published online 21 January 2008
- Subjects
UBIQUITIN; PROTEINS; INFLAMMATION; CARCINOGENESIS; HOMOLOGY (Biology)
- Publication
Oncogene, 2008, Vol 27, Issue 26, p3739
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1211042