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- Title
Characterization of Antisera to Paired Helical Filaments and Tau.
- Authors
Ihara, Yasuo; Kondo, Jun; Miura, Reiko; Nakagawa, Yasushi; Mori, Hiroshi; Honda, Toshiyuki
- Abstract
The two antisera to paired helical filaments (PHF) and tau, which stained neu-rofibrillary tangles similarly in tissue section, were characterized. Anti-PHF reacted exclusively with the carboxyl terminal portion of tau, whereas antitau reacted with the amino terminal portion. In the blots of PHF prepared in Sarkosyl (Sarkosyl-PHF), anti-PHF stained an extensive smear from the top to the bottom of the gel, while antitau did not stain this kind of smear but labeled a small amount of intact tau that was bound to Sarkosyl-PHF. An LDS-or SDS-insoluble residue of Sarkosyl-PHF was labeled with anti-PHF, but not with antitau. Antitau-defined epitopes were susceptible to protease, whereas anti-PHF-defined epitopes were unaffected. Based on these observations, we conclude that Sarkosyl-PHF were composed largely of the carboxyl terminal region of tau and accompanied by a small amount of intact tau on their surfaces. Copyright © 1990 S. Karger AG, Basel
- Publication
Gerontology, 1990, Vol 36, Issue S1, p15
- ISSN
0304-324X
- Publication type
Article
- DOI
10.1159/000213229