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- Title
Basal condensation of Numb and Pon complex via phase transition during Drosophila neuroblast asymmetric division.
- Authors
Zelin Shan; Yuting Tu; Ying Yang; Ziheng Liu; Menglong Zeng; Huisha Xu; Jiafu Long; Mingjie Zhang; Yu Cai; Wenyu Wen
- Abstract
Uneven distribution and local concentration of protein complexes on distinct membrane cortices is a fundamental property in numerous biological processes, including Drosophila neuroblast (NB) asymmetric cell divisions and cell polarity in general. In NBs, the cell fate determinant Numb forms a basal crescent together with Pon and is segregated into the basal daughter cell to initiate its differentiation. Here we discover that Numb PTB domain, using two distinct binding surfaces, recognizes repeating motifs within Pon in a previously unrecognized mode. The multivalent Numb-Pon interaction leads to high binding specificity and liquid-liquid phase separation of the complex. Perturbations of the Numb/Pon complex phase transition impair the basal localization of Numb and its subsequent suppression of Notch signaling during NB asymmetric divisions. Such phase-transition-mediated protein condensations on distinct membrane cortices may be a general mechanism for various cell polarity regulatory complexes.
- Subjects
PHASE transitions; DROSOPHILA; CELL polarity; PHASE separation; CELL division; CELL differentiation
- Publication
Nature Communications, 2018, Vol 9, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-018-03077-3