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- Title
Ecological Overlap and Horizontal Gene Transfer in Staphylococcus aureus and Staphylococcus epidermidis.
- Authors
Méric, Guillaume; Miragaia, Maria; de Been, Mark; Koji Yahara; Pascoe, Ben; Mageiros, Leonardos; Mikhail, Jane; Harris, Llinos G.; Wilkinson, Thomas S.; Rolo, Joana; Lamble, Sarah; Bray, James E.; Jolley, Keith A.; Hanage, William P.; Bowden, Rory; Maiden, Martin C. J.; Mack, Dietrich; de Lencastre, Hermínia; Feil, Edward J.; Corander, Jukka
- Abstract
The opportunistic pathogens Staphylococcus aureus and Staphylococcus epidermidis represent major causes of severe nosocomial infection, and are associated with high levels of mortality andmorbidity worldwide. These species are both commoncommensals on the human skin and in the nasal pharynx, but are genetically distinct, differing at 24% average nucleotide divergence in 1,478 core genes. To better understand the genome dynamics of these ecologically similar staphylococcal species, we carried out a comparative analysis of 324 S. aureus and S. epidermidis genomes, including 83 novel S. epidermidis sequences. A reference pan-genome approach andwhole genomemultilocus-sequence typing revealed that around half of the genomewas shared between the species. Based on a BratNextGen analysis, homologous recombination was found to have impacted on 40% of the core genes in S. epidermidis, but on only 24% of the core genes in S. aureus. Homologous recombination between the species is rare, with a maximum of nine gene alleles shared between any two S. epidermidis and S. aureus isolates. In contrast, there was considerable interspecies admixture of mobile elements, in particular genes associated with the SaPIn1 pathogenicity island, metal detoxification, and the methicillin-resistance island SCCmec. Our data and analysis provide a context for considering the nature of recombinational boundaries between S. aureus and S. epidermidis and, the selective forces that influence realized recombination between these species.
- Subjects
GENETIC transformation; STAPHYLOCOCCUS aureus; STAPHYLOCOCCUS epidermidis; NOSOCOMIAL infections; DETOXIFICATION (Alternative medicine)
- Publication
Genome Biology & Evolution, 2015, Vol 7, Issue 5, p1313
- ISSN
1759-6653
- Publication type
Article
- DOI
10.1093/gbe/evv066