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- Title
Evaluation of the human sperm nucleus: ambiguity and risk of confusion with chromomycin staining.
- Authors
Ménézo, Yves
- Abstract
Sperm chromatin structure assay high DNA stainability sperm as a marker of early miscarriage after intracytoplasmic sperm injection. When measured by aniline blue, the slight decrease observed was not found to be significant (Belloc I et al i ., 2009; Ménézo I et al i ., 2014b; Evenson I et al i ., 2020), coherent with a better padlocking of the brotamines. However, the dosage of 8-OHdG is delicate, as spontaneous oxidation of DNA may occur during processing (Badouard I et al. i , 2008; Aitken I et al. i , 2010) and this may lead to uncertain conclusions. Sperm head morphology is related to high deoxyribonucleic acid stainability assessed by sperm chromatin structure assay.
- Subjects
MALE infertility; SPERMATOZOA; SCIENTIFIC literature; DEMETHYLATION; PATERNAL age effect; DNA condensation; EMBRYOLOGY; INTRACYTOPLASMIC sperm injection
- Publication
Zygote, 2021, Vol 29, Issue 4, p257
- ISSN
0967-1994
- Publication type
Article
- DOI
10.1017/S0967199420000672