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- Title
计算机显微断层扫描技术 (μCT)在量化 阔叶树种导管中的应用.
- Authors
白春梅; 梁国芹; 符韵林; 韩尔康; 郭霞丽; 王 丰
- Abstract
Quantitative analysis of vessel characteristics at the cellular scale is of great significance for understanding plant adaptation strategies to environment. The direct grinding combined with stereo-microscope imaging is one of the main approaches to examine the anatomical structure of xylem (conifer tracheid and hardwood vessel) wood structure, which inevitably damages xylem cells, hindering the accurate understanding of anatomical structures. In this study, we applied X-ray micro-computed tomography (CT) and stereo-microscope technology to quantitatively measure the diameter and area of vessels of seven Canadian broadleaved tree species (Acer saccharum, Betula papyrifera, Fraxinus americana, Ostrya virginiana, Populus grandidentata, Quercus rubra, and Carya cordiformis). We fitted the results by linear model and tested the feasibility of µCT technology in quantifying the vessel size of broad- leaved species. We found that the results of the two methods for measuring vessel size were highly similar (R²= 0.98). The goodness of fit of the vessel diameter results measured by the two methods for the ring-porous wood species (C. cordiformis, R²=0.98; F. americana, R²=0.96; Q. rubra, R²=0.99) was higher than that of the diffuseporous wood species (B. papyrifera, R²=0.88; 0. virginiana, R²=0.73; A. saccharum, R²=0.68; P. grandidentata, R²=0.88). The goodness of fit of small vessels (diameter≤200 µm, R²=0.94) measured by the two methods was higher than that of large vessels (diameter>200 µm, R²=0.92). Thus, the µCT technique provided a new non-destructive detection method for quantifying xylem vessels of broadleaved tree species.
- Publication
Yingyong Shengtai Xuebao, 2024, Vol 35, Issue 5, p1214
- ISSN
1001-9332
- Publication type
Article
- DOI
10.13287/j.1001-9332.202405.008