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- Title
Phage Antibodies for Kanamycin Detection.
- Authors
Guliy, O. I.; Evstigneeva, S. S.; Staroverov, S. A.; Fomin, A. S.; Karavaeva, O. A.
- Abstract
Recombinant antibodies specific to kanamycin were obtained using a sheep display library of scFv fragments (Griffin.1) and the possibility of their use for the determination of kanamycin by dot-immunoassay was demonstrated. The minimum detectable concentration of kanamycin was 1 μg/mL (distinguishable label binding other than background). It has been shown that anti-kanamycin phage antibodies are specific for kanamycin and do not interact with other antibiotics (neomycin, tetracycline, ampicillin, and gentamicin). Anti-kanamycin phage antibodies are a promising alternative to monoclonal antibodies for use in the determination of kanamycin.
- Subjects
KANAMYCIN; RECOMBINANT antibodies; MONOCLONAL antibodies; BACTERIOPHAGES; NEOMYCIN; IMMUNOGLOBULINS
- Publication
Applied Biochemistry & Microbiology, 2023, Vol 59, Issue 5, p716
- ISSN
0003-6838
- Publication type
Article
- DOI
10.1134/S0003683823050071