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- Title
Cloning, Isolation, and Properties of a New Homologous Exoarabinase from the Penicillium canescens Fungus.
- Authors
Semenova, M. V.; Volkov, P. V.; Rozhkova, A. M.; Zorov, I. N.; Sinitsyn, A. P.
- Abstract
A novel exo-arabinase (GH93, exo-ABN) enzyme produced by the ascomycete Penicillium canescens has been studied. Cloning of the abn1 gene coding for exo-ABN into the recipient P. canescens strain RN3-11-7 yielded recombinant producing strains characterized by a high yield of extracellular exo- ABN production (20-30% of the total amount of extracellular protein). Chromatographic purification yielded a homogenous exo-ABN with a molecular weight of 47 kDa, as shown by SDS-PAGE. The enzyme showed high specific activity towards linear arabinan (117 U/mg) and low specific activity towards branched arabinan and arabinoxylan (4-5 U/mg) and para-nitrophenyl-α-L-arabinofuranoside (0.3 U/mg), whereas arabinogalactan and para-nitrophenyl-α-L-arabinopyranoside, the substrates that contained the pyranose form of arabinose, were not hydrolyzed. Arabinohexaose was the major product of linear arabinan hydrolysis. Exo-ABN had a pH optimum at 5.0 and a temperature optimum at 60°C. The enzyme was stable in a broad pH range (4.0-7.0) and upon heating to 50°C during 180 min. Extensive hydrolysis of linear and branched arabinans by exo- and endo-arabinase mixtures, arabinofuranosidase, and arabinofuran-arabinoxylan hydrolase has been performed. The degree of substrate conversion amounted to 67 and 83% of the maximal possible value, respectively.
- Subjects
ARABINASES; ENZYMES; PENICILLIUM; ARABINOXYLANS; NITROPHENYL compounds; BIOCHEMICAL substrates; HYDROLYSIS; ARABINOFURANOSIDASES
- Publication
Applied Biochemistry & Microbiology, 2018, Vol 54, Issue 4, p387
- ISSN
0003-6838
- Publication type
Article
- DOI
10.1134/S0003683818040130