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- Title
Characterization of altered regulation variants of dinitrogenase reductase-activating glycohydrolase from Rhodospirillum rubrum
- Authors
Kim, Kitai; Zhang, Yaoping; Roberts, Gary P.
- Abstract
In Rhodospirillum rubrum, nitrogenase activity is subject to posttranslational regulation through the adenosine diphosphate (ADP)-ribosylation of dinitrogenase reductase by dinitrogenase reductase ADP-ribosyltransferase (DRAT) and dinitrogenase reductase-activating glycohydrolase (DRAG). To study the posttranslational regulation of DRAG, its gene was mutagenized and colonies screened for altered DRAG regulation. Three different mutants were found and the DRAG variants displayed different biochemical properties including an altered affinity for divalent metal ions. Taken together, the results suggest that the site involved in regulation is physically near the metal binding site of DRAG.
- Subjects
RHODOSPIRILLUM rubrum; ADENOSINE diphosphate; GENETIC regulation; BINDING sites
- Publication
FEBS Letters, 2004, Vol 559, Issue 1-3, p84
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(04)00031-6