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- Title
Functional selectivity for glycerol of the nodulin 26 subfamily of plant membrane intrinsic proteins
- Authors
Wallace, Ian S.; Wills, David M.; Guenther, James F.; Roberts, Daniel M.
- Abstract
The nodulin-like intrinsic protein (NIP) subfamily of water and solute channels in plants is named for nodulin 26 of legume nodules. Two NIPs, soybean nodulin 26 and Lotus japonicus LIMP2, show a distinct functional profile with a low intrinsic osmotic water permeability (Pf) and the ability to flux uncharged polyols such as glycerol. NIPs have a conserved signature sequence within the ‘aromatic/arginine’ region that forms the selectivity filter for major intrinsic proteins. This sequence is a hybrid of glyceroporin and aquaporin residues as well as exhibiting substitutions unique to the NIP subfamily. Site-directed mutagenesis of a conserved tryptophan in helix 2 of LIMP2 shows that this is a major determinant of glycerol selectivity.
- Subjects
LEGUMES; NITROGEN fixation; OSMOSIS; TRYPTOPHAN
- Publication
FEBS Letters, 2002, Vol 523, Issue 1-3, p109
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)02955-1