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- Title
A thermosensitive mutant of HRV2 2A proteinase: evidence for direct cleavage of eIF4GI and eIF4GII
- Authors
Liebig, Hans-Dieter; Seipelt, Joachim; Vassilieva, Elena; Gradi, Alessandra; Kuechler, Ernst
- Abstract
Infection of mammalian cells with picornaviruses like entero-, rhino-, and aphthoviruses leads to an inhibition of cap-dependent cellular protein synthesis by the cleavage of both translation initiation factors, eIF4GI and eIF4GII. In entero- and rhinovirus infection this cleavage process is mediated by the viral 2A proteinase (2Apro). In order to discriminate between a direct mode of eIF4G cleavage and an indirect cleavage via activation of a cellular proteinase, a thermosensitive 2Apro mutant (ts-2Apro) of human rhinovirus 2 was employed. Temperature shift experiments of cytoplasmic HeLa cell extracts incubated with ts-2Apro strongly support a direct mode of cleavage of eIF4GI and eIF4GII by the viral 2Apro.
- Subjects
GENETIC mutation; RIBOSOMES; PROTEIN synthesis; RHINOVIRUSES
- Publication
FEBS Letters, 2002, Vol 523, Issue 1-3, p53
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)02933-2