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- Title
Recovery of campylobacter from human faeces stored at 4 °C.
- Authors
Ladrón de Guevara, C.; Pérez-Pomata, M. T.; Agulla, A.; Merino, F. J.; Villasante, P. A.; Velasco, A. C.
- Abstract
Six hundred and thirteen fresh diarrhoeal faeces were inoculated on Skirrow blood agar (SK), on Preston blood free agar (PBF), and in Campy-thioglycolate broth (CT). After 24 h of storage at 4 °C, specimens were again inoculated on SK and PBF, and in Campylobacter enrichment broth (CEB). CT tubes were placed overnight at 4 °C. Plates and CEB tubes were incubated at 43 °C in microaerophilic conditions. A total of 68 specimens was positive for campylobacter on direct plating. Sixty-four of them were also recovered after subculturing from CT, and only 51 from CEB. Delayed inoculation of plates after storage of samples at 4 °C yielded 57 isolates. The storage of faeces at 4 °C for 24 h significantly reduces the number of campylobacter isolates. When samples are not plated immediately we recommend inoculating a CT tube maintained at 4 °C overnight as a holding medium.
- Publication
Epidemiology & Infection, 1990, Vol 102, Issue 2, p281
- ISSN
0950-2688
- Publication type
Article
- DOI
10.1017/S0950268800029952