We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Survival of Escherichia coli O157:H7 in bottled natural mineral water.
- Authors
Kerr, M.; Fitzgerald, M.; Sheridan, J. J.; McDowell, D. A.; Blair, I. S.
- Abstract
M. KERR, M. FITZGERALD, J.J. SHERIDAN, D.A. MC DOWELL and I.S. BLAIR.1999.A non-verotoxin-producing isolate of Escherichia coli O157:H7 was inoculated at final concentrations of 103 or 106 ml-1 into natural non-carbonated mineral water (MW), sterile natural mineral water (SMW) and sterile distilled deionized water (SDDW) and stored at 15 °C for 10 weeks. Samples were examined every 7 d for the presence of E. coli O157:H7 using a resuscitative/selective agar procedure. The MW samples were also plated onto a non-selective agar, R2A, to enumerate E. coli O157:H7 and the autochthonous flora. There was a significant difference in the survival of E. coli O157:H7 (103 ml-1 inoculum) between the MW and the SDDW at time periods 0, 7, 14 (P < 0·005) 21, 28, 35 (P < 0·001) and 42 d (P < 0·05) and between the MW and the SMW at time periods 7, (P < 0·05) 14, 21 (P < 0·005) 28 (P < 0·01) and 35 d (P < 0·05), with the pathogen surviving longest in the MW samples. In contrast, at 106 ml-1, no significant differences in the survival of E. coli O157:H7 were observed between the water types. The presence of E. coli O157:H7 (103 ml-1) in the MW samples did not have an antagonistic effect on the recovery of the autochthonous flora. Transmission electron microscopy analysis demonstrated that the E. coli O157:H7 cells lyse during storage, releasing their contents into the surrounding environment. These substances may have been utilized by the autochthonous flora and thereby explain why the numbers of flora recovered from the inoculated MW samples were higher than those recovered from the uninoculated samples.
- Subjects
NORTHERN Ireland; ESCHERICHIA coli; MINERAL water bottles; MINERAL water biology
- Publication
Journal of Applied Microbiology, 1999, Vol 87, Issue 6, p833
- ISSN
1364-5072
- Publication type
Article
- DOI
10.1046/j.1365-2672.1999.00928.x