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- Title
Specificity of Rabbit Antisera against Mouse Leukaemias <em>in vitro</em>.
- Authors
Reif, A. E.; Kim, Chung-ai H.
- Abstract
Rabbit antisera were prepared against mouse leukaemias from several inbred strains, and against a mixture of these leukaemias. The potencies of these antisera were tested against each of the different leukaemias, and against certain normal cells and tissues. As determined by immune cytolysis, the potencies of antisera were strongest against the homologous leukaemias. The potencies against unrelated leukaemias were generally within 50 per cent of those against the homologous leukaemias, with some exceptions. Antisera prepared by injection of a mixture of the leukaemia cells showed remarkably high potencies against all leukaemias tested. As measured by C fixation, the same antisera showed less specificity for the homologous leukaemias. The potencies of antisera for C fixation on a variety of normal tissues was quite strong, and decreased approximately in the following order: testis, kidney, liver, lung, brain, muscle, erythrocytes. For antiserum prepared against DBA/2 leukaemia L1210, the C fixation reaction against normal lymph node lymphocytes was about 20 per cent as strong as the reaction against the homologous leukaemia L1210. A hypothesis concerning the mechanism of immune cystolysis is presented to explain these results. Exhaustive absorption of anti-L1210 sera with normal DBA/2 tissues produced only a slight increase in antileukaemia specificity.
- Subjects
IMMUNE serums; LEUKEMIA immunology; IMMUNOSPECIFICITY; CELLULAR immunity; IMMUNITY
- Publication
Immunology, 1971, Vol 20, Issue 6, p1087
- ISSN
0019-2805
- Publication type
Article