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- Title
Circulating EGFR Mutations in Patients with Lung Adenocarcinoma by Circulating Tumor Cell Isolation Systems: A Concordance Study.
- Authors
Li, Shih-Hong; Wu, Min-Hsien; Wang, Hung-Ming; Hsu, Ping-Chih; Fang, Yueh-Fu; Wang, Chih-Liang; Chu, Hui-Chun; Lin, Hung-Chih; Lee, Li-Yu; Wu, Ching-Yang; Yang, Cheng-Ta; Chen, Jen-Shi; Hsieh, Jason Chia-Hsun
- Abstract
Background: We developed a hybrid platform using a negative combined with a positive selection strategy to capture circulating tumor cells (CTCs) and detect epidermal growth factor receptor (EGFR) mutations in patients with metastatic lung adenocarcinoma. Methods: Blood samples were collected from patients with pathology-proven treatment-naïve stage IV lung adenocarcinoma. Genomic DNA was extracted from CTCs collected for EGFR mutational tests. The second set of CTC-EGFR mutational tests were performed after three months of anti-cancer therapy. Results: A total of 80 samples collected from 28 patients enrolled between July 2016 and August 2018. Seventeen patients had EGFR mutations, including Exon 19 deletion (n = 11), L858R (n = 5), and de-novo T790 and L858R (n = 1). Concordance between tissue and CTCs before treatment was 88.2% in EGFR- mutant patients and 90.9% in non-mutant patients. The accuracy, sensitivity, specificity, positive predictive value, and negative predictive value of EGFR mutation tests for CTCs were 89.3%, 88.2%, 90.9%, 93.8%, and 83.3%, respectively. Conclusions: CTCs captured by a hybrid platform using a negative and positive selection strategy may serve as a suitable and reliable source of lung cancer tumor DNA for detecting EGFR mutations, including T790M.
- Subjects
LUNGS; CELL separation; EPIDERMAL growth factor receptors
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 18, pN.PAG
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms231810661