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- Title
High-Level Production of a Monoclonal Antibody in Murine Myeloma Cells by Perfusion Culture Using a Gravity Settler.
- Authors
Chiou-Yu Choo; Yuan Tian; Wan-Seop Kim; Erich Blatter; Jon Conary; Ciaran P. Brady
- Abstract
A perfusion system is described for the production of a human monoclonal antibody in non-secreting murine myeloma (NS0) cells that was previously shown to be difficult to produce at high levels using fed-batch culture. The perfusion system was based on the use of a commercially available cell settler as the separation device to separate the cells from the culture. Separation efficiency of the cell settler was above 98%. Based on the growth and glucose consumption rates, fresh media was added to the culture and the turnover rate for the bioreactor was set at a maximum of 1.5 times the bioreactor volume per day. The perfusion process resulted in twice the maximum viable cell densities and up to three times the total protein production in a 53-day run period when compared to the fed-batch process. In addition, charge heterogeneity of the antibody as measured by ion exchange chromatography was lower for material purified from the perfusion runs compared to fed-batch. Perfusion mode of culture using a commercially available gravity settler is therefore a viable alternative to fed-batch mode for high-level production of this monoclonal antibody in NS0 cells.
- Subjects
MONOCLONAL antibodies; PERFUSION; ION exchange chromatography; BIOREACTORS
- Publication
Biotechnology Progress, 2007, Vol 23, Issue 1, p225
- ISSN
8756-7938
- Publication type
Article
- DOI
10.1021/bp060231v