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- Title
Effects of non-iron metalloporphyrins on growth and gene expression of Porphyromonas gingivalis.
- Authors
Yukitake, Hideharu; Naito, Mariko; Sato, Keiko; Shoji, Mikio; Ohara, Naoya; Yoshimura, Mamiko; Sakai, Eiko; Nakayama, Koji
- Abstract
The oral anaerobic bacterium Porphyromonas gingivalis, which is implicated as an important pathogen for chronic periodontitis, requires heme for its growth. Non-iron metalloporphyrins, In-PPIX and Ga-PPIX, were examined for antibacterial effects on P. gingivalis. Both In-PPIX and Ga-PPIX caused retardation of P. gingivalis growth in a dose-dependent fashion. Microarray and qPCR analyses revealed that In-PPIX treatment upregulated the expression of several genes encoding proteins including ClpB and ClpC, which are members of the Clp (caseinolytic protease, Hsp100) family, and aRNR, aRNR-activating protein and thioredoxin reductase, whereas In-PPIX treatment had no effect on the expression of genes encoding proteins involved in heme uptake pathways, Hmu-mediated, Iht-mediated and Tlr-mediated pathways. P. gingivalis ihtA and ihtB mutants were more resistant to In-PPIX than was the wild-type parent, whereas hmuR and tlr mutants did not show such resistance to In-PPIX. The results suggest that In-PPIX is incorporated by the Iht-mediated heme uptake pathway and that it influences protein quality control and nucleotide metabolism and retards growth of P. gingivalis.
- Subjects
PORPHYROMONAS gingivalis; ANAEROBIC bacteria growth; ANAEROBIC bacteria genetics; DRUG efficacy; PORPHYRINS; POLYMERASE chain reaction; PROTEIN microarrays
- Publication
Microbiology & Immunology, 2011, Vol 55, Issue 3, p141
- ISSN
0385-5600
- Publication type
Article
- DOI
10.1111/j.1348-0421.2010.00299.x