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- Title
Characterization of theDrosophila Methoprene -tolerant gene product.
- Authors
Miura, Ken; Oda, Masahito; Makita, Sumiko; Chinzei, Yasuo
- Abstract
Juvenile hormones (JHs) of insects are sesquiterpenoids that regulate a great diversity of processes in development and reproduction. As yet the molecular modes of action of JH are poorly understood. TheMethoprene-tolerant(Met) gene ofDrosophila melanogasterhas been found to be responsible for resistance to a JH analogue (JHA) insecticide, methoprene. Previous studies onMethave implicated its involvement in JH signaling, although direct evidence is lacking. We have now examined the product ofMet(MET) in terms of its binding to JH and ligand-dependent gene regulation.In vitrosynthesized MET directly bound to JH III with high affinity (Kd = 5.3 ± 1.5 nm, mean ± SD), consistent with the physiological JH concentration. In transient transfection assays usingDrosophilaS2 cells the yeast GAL4-DNA binding domain fused to MET exerted JH- or JHA-dependent activation of a reporter gene. Activation of the reporter gene was highly JH- or JHA-specific with the order of effectiveness: JH III » JH II > JH I > methoprene; compounds which are only structurally related to JH or JHA did not induce any activation. Localization of MET in the S2 cells was nuclear irrespective of the presence or absence of JH. These results suggest that MET may function as a JH-dependent transcription factor.
- Subjects
JUVENILE hormones; DROSOPHILA; GENES; HORMONES; GENETIC transformation; TRANSCRIPTION factors
- Publication
FEBS Journal, 2005, Vol 272, Issue 5, p1169
- ISSN
1742-464X
- Publication type
Article
- DOI
10.1111/j.1742-4658.2005.04552.x