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- Title
RNA splicing promotes translation and RNA surveillance.
- Authors
Gudikote, Jayanthi P; Imam, J Saadi; Garcia, Ramon F; Wilkinson, Miles F
- Abstract
Aberrant mRNAs harboring premature termination codons (PTCs or nonsense codons) are degraded by the nonsense-mediated mRNA decay (NMD) pathway. mRNAs transcribed from genes that naturally acquire PTCs during lymphocyte development are strongly downregulated by PTCs. Here we show that a signal essential for this robust mRNA downregulatory response is efficient RNA splicing. Strong mRNA downregulation can be conferred on a poor NMD substrate by either strengthening its splicing signals or removing its weak introns. Efficient splicing also strongly promotes translation, providing a molecular explanation for enhanced NMD and suggesting that efficient splicing may have evolved to enhance both protein production and RNA surveillance. Our results suggest simple approaches for increasing protein expression from expression vectors and treating human genetic diseases caused by nonsense and frameshift mutations.
- Subjects
RNA splicing; GENETIC regulation; BIOSYNTHESIS; CELLULAR control mechanisms; MOLECULAR genetics; GENE expression
- Publication
Nature Structural & Molecular Biology, 2005, Vol 12, Issue 9, p801
- ISSN
1545-9993
- Publication type
Article
- DOI
10.1038/nsmb980