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- Title
Phytodecta fornicata Brüggemann resistance mediated by oryzacystatin II proteinase inhibitor transgene.
- Authors
Slavica NinkoviÄ; Jovanka MiljuÅ¡-ÄukiÄ; Svetlana RadoviÄ; Vesna MaksimoviÄ; Jelica LazareviÄ; Branka Vinterhalter; Mirjana NeÅ¡koviÄ; Ann Smigocki
- Abstract
Abstract  Phytodecta fornicata Br�ggemann is a serious pest of alfalfa (Medicago sativa L.) that causes significant crop loss in the Balkan peninsula of Europe. We introduced a wound-inducible oryzacystatin II (OCII) gene to alfalfa to evaluate its effect on survival of P. fornicata larvae. Feeding bioassays with second, third and fourth instars were carried out using transgenic plants that were shown to express OCII at 24 and 48 h after wounding. Second and third instars were the most sensitive to the ingestion of OCII, whereas no effects were observed with fourth instars. About 80% of the second and third instars died after 2 days of feeding on the transgenic plants as compared to 0â40% on the controls. This is the first report that demonstrates significant increase in mortality of P. fornicata on transgenic plants that express a cysteine proteinase inhibitor gene, and this knowledge should lead to the development of effective management strategies for this devastating pest of alfalfa.
- Subjects
ALFALFA diseases &; pests; PLANT genetic engineering; MEDICAGO; ABSORPTION (Physiology)
- Publication
Plant Cell, Tissue & Organ Culture, 2007, Vol 91, Issue 3, p289
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-007-9296-2