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- Title
Enhancement of Antiproliferative and Proapoptotic Effects of Cadmium Chloride Combined with hSmac in Hepatocellular Carcinoma Cells.
- Authors
Guo, Caixia; Li, Yanbo; Zhang, Haixia; Wang, Zhicheng; Jin, Minghua; Zhang, Long; An, Liping; Hu, Guiqin; Liu, Xiaomei; Liu, Ying; Du, Haiying; Sun, Zhiwei
- Abstract
Background: To study the effects of cadmium chloride (CdCl2) combined with hSmac on the proliferation and apoptosis of hepatocellular carcinoma cells, i.e. SMMC-7721. Methods: SMMC-7721 cells were transfected with pcDNA3.1+-hSmac using a lipofectamine-mediated method, and then cell viability was detected by MTT assay after exposure to 10, 20, and 30 μmol/l CdCl2. Apoptosis was determined by both acridine orange-ethidium bromide staining and flow cytometry, and expressions of caspase-3, caspase-9, and cytochrome c by Western blot. Results: CdCl2 had cytotoxicity to SMMC-7721 cells, and it could inhibit proliferation in a dose-dependent manner and induce apoptosis; hSmac could inhibit proliferation and induce apoptosis independently in SMMC-7721 cells. Furthermore, cotreatment with CdCl2 and hSmac could enhance antiproliferative and proapoptotic effects in SMMC-7721 cells. Conclusions: hSmac could enhance the cytotoxicity of CdCl2. Copyright © 2011 S. Karger AG, Basel
- Subjects
LIVER cancer; CADMIUM chloride; APOPTOSIS; MYOBLAST transfer therapy; FLOW cytometry; CYTOCHROME c; WESTERN immunoblotting; COMBINATION drug therapy
- Publication
Chemotherapy (0009-3157), 2011, Vol 57, Issue 1, p27
- ISSN
0009-3157
- Publication type
Article
- DOI
10.1159/000321031