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- Title
Correcting spherical aberrations in a biospecimen using a transmissive liquid crystal device in two-photon excitation laser scanning microscopy.
- Authors
Ayano Tanabe; Terumasa Hibi; Sari Ipponjima; Kenji Matsumoto; Masafumi Yokoyama; Makoto Kurihara; Nobuyuki Hashimoto; Tomomi Nemoto
- Abstract
Two-photon excitation laser scanning microscopy has enabled the visualization of deep regions in a biospecimen. However, refractive-index mismatches in the optical path cause spherical aberrations that degrade spatial resolution and the fluorescence signal, especially during observation at deeper regions. Recently, we developed transmissive liquid-crystal devices for correcting spherical aberration without changing the basic design of the optical path in a conventional laser scanning microscope. In this study, the device was inserted in front of the objective lens and supplied with the appropriate voltage according to the observation depth. First, we evaluated the device by observing fluorescent beads in single- and two-photon excitation laser scanning microscopes. Using a 25× water-immersion objective lens with a numerical aperture of 1.1 and a sample with a refractive index of 1.38, the device recovered the spatial resolution and the fluorescence signal degraded within a depth of ±0.6 mm. Finally, we implemented the device for observation of a mouse brain slice in a two-photon excitation laser scanning microscope. An optical clearing reagent with a refractive index of 1.42 rendered the fixed mouse brain transparent. The device improved the spatial resolution and the yellow fluorescent protein signal within a depth of 0-0.54 mm.
- Subjects
LIQUID crystal devices; SPHERICAL aberration; ADAPTIVE optics; PHASE modulation; LABORATORY mice
- Publication
Journal of Biomedical Optics, 2015, Vol 20, Issue 10, p101204-1
- ISSN
1083-3668
- Publication type
Article
- DOI
10.1117/1.JBO.20.10.101204