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- Title
Injectable dECM-enhanced hyaluronic microgels with spatiotemporal release of cartilage-specific molecules to improve osteoarthritic chondrocyte's function.
- Authors
Deng, Siyan; Cao, Hongfu; Lu, Yan; Shi, Wenqing; Chen, Manyu; Cui, Xiaolin; Liang, Jie; Fan, Yujiang; Wang, Qiguang; Zhang, Xingdong
- Abstract
The interior environment of articular cartilage in osteoarthritis (OA) presents substantial hurdles, leading to the malfunction of chondrocytes and the breakdown of collagen II-enriched hyaline cartilage matrix. Despite this, most clinical treatments primarily provide temporary relief from OA discomfort without arresting OA progression. This study aimed to alleviate OA by developing intra-articular injectable dECM-enhanced hyaluronic (HE) microgels. The HE hydrogel was engineered and shaped into uniformly sized microgels using microfluidics and photopolymerization techniques. These microgels provided a spatiotemporal cascade effect, facilitating the rapid release of growth factors and a slower release of ECM macromolecules and proteins. This process assisted in the recovery of OA chondrocytes' function, promoting cell proliferation, matrix synthesis, and cartilage-specific gene expression in vitro. It also effectively aided repair of the collagen II-enriched hyaline cartilage and significantly reduced the severity of OA, as demonstrated by radiological observation, gross appearance, histological/immunohistochemical staining, and analysis in an OA rat model in vivo. Collectively, the HE injectable microgels with spatiotemporal release of cartilage-specific molecules have shown promise as a potential candidate for a cell-free OA therapy approach.
- Subjects
MICROGELS; LABORATORY rats; ARTICULAR cartilage; MOLECULES; IMMUNOSTAINING; POLYVINYL alcohol
- Publication
Collagen & Leather, 2024, Vol 6, Issue 1, p1
- ISSN
2731-6998
- Publication type
Article
- DOI
10.1186/s42825-024-00158-6