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- Title
いわゆる健康食品中に含有する植物遺伝子検出のための DNA 希釈法を用いたPCR 条件及び解析条件に関する検討
- Authors
小澤広規; 髙橋美津子; 菅谷なえ子; 桜井克巳; 荒金眞佐子; 高橋直矢; 宇宿秀三; 斉藤貢一
- Abstract
The conditions for detecting plant-derived genes and identifying plant species in health teas, supplements, and so-called health foods in the polymerase chain reaction (PCR) were examined. The results showed that the genes may not be amplified in many samples because plant fragments and supplements may contain substances that inhibit PCR. Gene amplification by PCR was realized by suppressing the effect of inhibitors through the dilution of DNA extract of samples with nuclease-free water at a certain dilution rate. On the other hand, use of the rbcLa_F/rbcL-475R primer set, which is a combination of primers indicated by the Plant Working Group and primers prepared in this study, in PCR of the rbcL region yielded a single sequence waveform with high sensitivity and accuracy, which made it possible to analyze genes with longer sequences than usual. Applying this method to the genetic analysis of weight loss (slimming type) foods enabled us to detect the genes of Senna alexandrina and Cassia alata, which are laxatives, in the foods whose plant ingredients cannot be identified morphologically.
- Publication
Japanese Journal of Food Chemistry & Safety, 2019, Vol 26, Issue 1, p28
- ISSN
1341-2094
- Publication type
Article