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- Title
Sperm DNA fragmentation and reproductive outcomes.
- Authors
Bajaj, Bindu; Kapoor, Garima
- Abstract
In the last few years, male infertility is increasingly catching the attention of researchers. The impact of spermatozoal factors on the fertilization and early embryo development is being deciphered. An association of sperm aneuploidy status in unexplained recurrent pregnancy loss has been observed. Sperm DNA damage or the sperm DNA fragmentation (sDF) is now measurable as DNA fragmentation index. The methods of evaluating sDF are many and they differ in their technique and paraphernalia needed to perform them. The most common methods are SCSA, TUNEL, SCD, COMET. TUNEL utilizes tagging of DNA in places where there is a break and then quantifying them. SCD is based on the principal of susceptibility of fragmented DNA to acid denaturation. COMETassay involves variable movement of intact and fragmented DNA under the influence of electric field. There are different methods to perform each of the previous mentioned tests though the principle remains the same. Each laboratory performing these tests needs to standardize them and should have their own cutoffs, which should relate to the procedure [intrauterine insemination/in vitro fertilization (IVF)/intracytoplasmic sperm injection] being performed. The role of sDF is slowly getting defined in recurrent implantation failures, unexplained infertility, varicocele, and IVF failures. Yet the jury is still out.
- Subjects
SPERMATOZOA analysis; EVALUATION of medical care; CONCEPTION; SEMEN analysis; HUMAN reproductive technology; HUMAN artificial insemination; DNA damage; FERTILIZATION in vitro; REPRODUCTIVE health
- Publication
Fertility Science & Research, 2022, Vol 9, Issue 1, p10
- ISSN
2394-4285
- Publication type
Article
- DOI
10.4103/fsr.fsr_49_21