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- Title
MOLECULAR CHARACTERIZATION OF RECOMBINANT AND NON RECOMBINANT COTTON VARITIES OF COMMERCIAL IMPORTANCE.
- Authors
Asree, Hala Jumaah; Falih, Aysar Ihsan; Mahdi, Dalya Salah; Kadhim AL-Maameri, Ahmed Adnan; Khirallah, Ahmed Ali
- Abstract
An extended multiplex PCR method was established to rapidly identify and classify Bacillus thuringiensis strains containing cry (crystal protein) genes toxic to species of Lepidoptera, Coleoptera, and Diptera. The technique enriches current strategies and simplifies the initial stages of large-scale screening of cry genes by pinpointing isolates that contain specific genes or unique combinations of interest with potential insecticidal activities, thus facilitating subsequent toxicity assays. For this assay we selected a pair of Cry 1 gene(npt II) and differentiated the BT-cotton and non Bt-cotton. And we identified that this Bt-cotton is rapidly infected or toxic to species of Lepidoptera, Coleoptera, and Diptera.
- Subjects
COTTON varieties; BACILLUS thuringiensis; LEPIDOPTERA; COTTON genetics; BIOLOGICAL assay
- Publication
Biochemical & Cellular Archives, 2019, Vol 19, Issue 2, p3055
- ISSN
0972-5075
- Publication type
Article
- DOI
10.35124/bca.2019.19.2.3055