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- Title
PREVALENCE OF ANTIBIOTIC RESISTANCE IN THE BACTERIAL ISOLATES SOURCED FROM DISEASED FISH OF DIFFERENT ENVIRONMENTAL CONDITIONS OF SUB HIMALAYAN REGIONS.
- Authors
G., Suneeta; Prasad, Yogendra
- Abstract
This study was conducted to know the prevalence of antibiotic resistance among the isolates of A. hydrophila of different ecological conditions viz. a reservoir (Nanak Sagar, UK), river (Nakatia at Bareilly fed with the Himalayan rivers) and fish stocking ponds (Puranpur fish hatchery) found persistently associated with diseased Labeo rohita, Cirrhinus mrigala, Calta catla and Cyprinus carpio with explicit symptoms of haemorrhagic septicaemia (BHS). Out of 28 isolates of bacteria isolated on Rimler Shotts (RS) medium followed by MacConkey agar, AIA and Nutrient agar (Hi Media, Mumbai) and subjected to physical (temp. pH and salinity), biochemical tests (positive response to oxidase, catalase, gelatin, nitrate utilization, H2S production, Indole, VP reaction, oxidative fermentative tests, blood agar, citrate and negative to methyle red and urease), 15 isolates (54%) were confirmed as A. hydrophila along with ATCC 7966. There were variations in the antibiotic resistance pattern (checked using octadiscs of 23 antibiotics) as the very few (20%) isolates of A. hydrophila of fish of Nanak Sagar exhibited mild to moderate resistance to novobiocin, amoxyclave whereas the 100% isolates of Nakatia river found to be resistant to novobiocin, amoxyclave followed by ampicillin, co-trimoxazole (90%), sulphatrid, teioplanin, co-trimazine, co-trimoxazole -sulpha/trimethoprim (80%), colistin sulphate (60%), cephalothin, nitrofurantoin, tetracyclin (40%), streptomycin, gentamicin, netillin and ceftriaxone (20%). Interestingly, majority of the isolates of Puranpur fish hatchery were resistant to novobiocin, amoxyclave, ampicillin, sulphatrid and teioplanin. None of the isolates exhibited resistance to amikacin, kanamycin, carbenicillin, levofloxacin, ofloxacin, chloramphenicol and ciprofloxacin. It can be concluded that A. hydrophila of varied environmental conditions showed different pattern of antibiotic resistance, more in reverine and hatchery systems and less in reservoir. If it prevails there may be the chance of gene transfer among other groups of bacteria which may prove to be fatal to aquaculture industries of Sub Himalayan regions.
- Subjects
AEROMONAS hydrophila; AQUACULTURE; DRUG resistance; NOVOBIOCIN; SEPSIS
- Publication
Biochemical & Cellular Archives, 2019, Vol 19, Issue 1, p531
- ISSN
0972-5075
- Publication type
Article
- DOI
10.35124/bca.2019.19.1.531