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- Title
氮素水平对潮土氨氧化微生物和硝化作用的影响.
- Authors
陈照明; 王强; 李燕丽; 张金萍; 冯江; 刘涛; 俞巧钢; 马军伟
- Abstract
In order to clarify the effects of different nitrogen levels on soil nitrification and ammonia oxidizing microorganisms, especially the response specificity of ammonia oxidizing bacteria (AOB) and ammonia oxidizing archaea (AOA) under high nitrogen levels, fluvo-aquic soil was used as the test soil. Using urea and ammonium sulfate as nitrogen sources, set five nitrogen levels (in terms of N, 0, 150, 300, 600, 1200 mg·kg-1, respectively, and correspondingly, denoted as N0, N150, N300, N600, N1200), 28 days of microcosm culture was carried out to study the effects of urea and ammonium sulfate on soil AOB, AOA abundance and soil nitrification under different nitrogen levels. The results showed that the addition of nitrogen fertilizer significantly (P<0.05) decreased soil pH At the end of cultivation (28 d), soil pH value decreased with the increase of nitrogen level. At the end of cultivation, soil NH4+-N content of each nitrogen addition treatment was at a low level (0.72-2.01 mg·kg-1 ). Compared with N0 treatment, the addition of urea or ammonium sulfate increased the copy number of AOB amoA gene, and the copy number of AOB amoA gene increased with the increase of nitrogen level; but the application of nitrogen fertilizer had no significant effect on the copy number of AOA amoA gene. The soil average The net nitrification rate increased with the increase of nitrogen level, and under the same nitrogen level, the average net nitrification rate of soil treated with urea was higher than that of ammonium sulfate treatment. The correlation analysis showed that soil NO2--N+NO3--N content was significantly related to soil NO2--N+NO3--N content. AOB amoA gene copy number showed a significant (P<0.05) positive correlation, but no significant correlation with AOA amoA gene copy number, indicating that AOB played a leading role in the nitrification of fluvo-aquic soil. In conclusion, high nitrogen levels did not inhibit fluvo-aquic soil. The nitrification of AOB promotes soil nitrification instead, and the process is mainly dominated by AOB. The research results can provide a theoretical basis for the scientific management of nitrogen fertilizer on fluvo-aquic soil.
- Subjects
AMMONIA-oxidizing bacteria; UREA as fertilizer; NITROGEN fertilizers; AMMONIUM sulfate; NITROGEN in soils; SOIL acidity
- Publication
Acta Agriculturae Zhejiangensis, 2022, Vol 34, Issue 9, p2004
- ISSN
1004-1524
- Publication type
Article
- DOI
10.3969/j.issn.1004-1524.2022.09.19