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- Title
Control of Locomotory Behavior of Caenorhabditis elegans by the Immunoglobulin Superfamily Protein RIG-3.
- Authors
Bhardwaj, Ashwani; Pandey, Pratima; Babu, Kavita
- Abstract
Cell surface immunoglobulin superfamily (IgSF) proteins play important roles in the development and function of the nervous system. Here we define the role of a Caenorhabditis elegans IgSF protein, RIG-3, in the function of the AVA command interneuron. This study reveals that RIG-3 regulates the abundance of the glutamate receptor subunit, GLR-1, in the AVA command interneuron and also regulates reversal behavior in C. elegans. The mutant strain lacking rig-3 (rig-3 (ok2156)) shows increased reversal frequency during local search behaviors. Genetic and behavioral experiments suggest that RIG-3 functions through GLR-1 to regulate reversal behavior. We also show that the increased reversal frequency seen in rig-3 mutants is dependent on the increase in GLR-1 abundance at synaptic inputs to AVA, suggesting that RIG-3 alters the synaptic strength of incoming synapses through GLR-1. Consistent with the imaging experiments, altered synaptic strength was also reflected in increased calcium transients in rig-3 mutants when compared to wild-type control animals. Our results further suggest that animals lacking rig-3 show increased AVA activity, allowing the release of FLP-18 neuropeptide from AVA, which is an activity-dependent signaling molecule. Finally, we show that FLP-18 functions through the neuropeptide receptor, NPR-5, to modulate reversal behavior in C. elegans.
- Subjects
CALCIUM metabolism; NEURAL physiology; CELL receptors; ANIMAL behavior; ANIMAL experimentation; CELLULAR signal transduction; GENETICS; GENETIC techniques; HUMAN locomotion; IMMUNOGLOBULINS; GENETIC mutation; NEMATODES; NEUROPEPTIDES; NEUROPHYSIOLOGY; CELL physiology
- Publication
Genetics, 2020, Vol 214, Issue 1, p135
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.119.302872