We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Di (2‐ethylhexyl) phthalate induces cytotoxicity in HEK‐293 cell line, implication of the Nrf‐2/HO‐1 antioxidant pathway.
- Authors
Amara, Ines; Timoumi, Rim; Graiet, Imen; Ben Salem, Intidhar; Adelou, Kamilath; Abid‐Essefi, Salwa
- Abstract
The di (2‐ethylhexyl) phthalate (DEHP) is a plasticizer used in the polyvinyl chloride industry. Human exposure to this plasticizer is inevitable and contributes to several side effects. In this study, we examined whether DEHP induces apoptosis and oxidative stress in embryonic kidney cells (HEK‐293) and whether the nuclear factor E2‐related factor 2 (Nrf‐2)/heme oxygenase‐1 (HO‐1) antioxidant pathway is involved in the pathogenesis of this process. We demonstrated that DEHP is cytotoxic to HEK‐293 cells. It causes oxidative damage through the generation of free radicals, induces lipid peroxidation, and alters superoxide dismutase and catalase activities. Simultaneously, DEHP treatment decreases the expression and the protein level of Nrf‐2 and HO‐1. Inhibition of the Nrf‐2/HO‐1 pathway is related to the mitochondrial pathway of apoptosis. This apoptotic process is characterized by a loss of mitochondrial transmembrane potential (ΔΨm) and upregulation of the expression of caspase‐3 mRNA as well as its protein level.
- Subjects
PLASTICIZERS; POLYVINYL chloride industry; CELL lines; MEMBRANE potential; SUPEROXIDE dismutase; OXIDATIVE stress
- Publication
Environmental Toxicology, 2019, Vol 34, Issue 9, p1034
- ISSN
1520-4081
- Publication type
Article
- DOI
10.1002/tox.22774