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- Title
Bcl-xL DNAzymes promote radiosensitivity and chemosensitivity in colorectal cancer cells via enhancing apoptosis.
- Authors
Yu, Zhen; Guo, Jun; Meng, Tao; Ge, Lei; Liu, Lin; Wang, Haijiang; Yang, Xinhui
- Abstract
Background: RNA-cleaving deoxyribozymes (DNAzymes) are catalytic deoxyribonucleic acid molecules that have become a promising new class of gene suppressors by binding and cleaving target mRNA. This study investigated whether DNAzymes targeting Bcl-xL enhanced the effectiveness of radiotherapy and chemotherapy in colorectal cancer (CRC) cells. Methods: Two types of CRC cells, SW480 and SW837, were transfected with five DNAzymes. Cell viability, Bcl-xL expression and apoptosis were examined. SW480 xenograft model was used to examine the combined effects of Bcl-xL DNAzymes and 5-FU (or X-rays) on tumor growth. Results: Three Bcl-xL DNAzymes, DT882, DT883, and DT884 were identified to be effective in suppressing Bcl-xL expression and causing cell apoptosis. Furthermore, DT882 combined with 5-FU or radiotherapy addictively promoted cell apoptosis and significantly inhibited the growth of SW480 xenografts in vivo. Conclusions: These results suggest that Bcl-xL DNAzymes can enhance the radiosensitivity and chemosensitivity in CRC cells via inducing apoptosis.
- Subjects
DEOXYRIBOZYMES; COLORECTAL cancer; DNA; RADIATION tolerance; APOPTOSIS; CANCER cells
- Publication
BMC Pharmacology & Toxicology, 2022, Vol 23, Issue 1, p1
- ISSN
2050-6511
- Publication type
Article
- DOI
10.1186/s40360-022-00553-x