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- Title
Elucidating S-allyl-L-cysteine Impact to the Heart Function and Vascular Reactivity in Estrogen-deficiency Rats.
- Authors
Zainalabidin, Satirah; Abdul Ghani, Muhamad Adib; Jie, Lee; Mahadi, Mohd Kaisan; Muhammad, Norliza
- Abstract
Introduction: Ischemic heart disease is more prevalent among postmenopausal women due to estrogen deprivation. Estrogen is deemed as cardioprotective as it regulates vascular function by eliciting the release of nitric oxide (NO). S-Allyl-L-cysteine (SAC), a compound found abundantly in aged garlic, is believed to protect against myocardial ischemia-reperfusion injury (IRI) and vascular dysfunction by stimulating the production of hydrogen sulfide (H2S), a gasotransmitter with a similar function as NO. This study aims to explore the impact of SAC in myocardial IRI model in ovariectomized rats. Methods: Thirty female Wistar rats were randomly allocated into five groups: Sham, OVX+Sham-IR+SAC, OVX+IR, OVX+IR+SAC+PAG (propargylglycine, CSE inhibitor) and OVX+IR+SAC. Rats underwent bilateral ovariectomy through a ventral approach, while sham-ovariectomy rats underwent the same procedure without cutting the ovaries. After recovery, the rat’s heart was isolated and underwent 45 min ischemia and 120 min reperfusion with SAC (1 mM) treatment in the first 15 min of reperfusion. Throughout the experiment, heart function was recorded, and coronary effluent was measured at different time points for lactate dehydrogenase, and the infarct size was analyzed. Simultaneously, the thoracic aorta was isolated to measure for vascular reactivity. Results: Ovariectomized rats exhibited cardiac damage from increased troponin-T in the blood (p<0.05). SAC improved the left ventricle developed pressure following an ischemic insult and significantly reduced total LDH release and infarct size (p<0.05). Endothelium-dependent relaxation induced by acetylcholine of the ovariectomized rat’s aorta was improved after incubation with SAC for 1 hour. Conclusion: SAC exerted cardioprotective in preventing detrimental effects after myocardial ischemia injury.
- Subjects
MYOCARDIAL reperfusion; CORONARY disease; RATS; MYOCARDIAL ischemia; HEART; LABORATORY rats
- Publication
Malaysian Journal of Medicine & Health Sciences, 2024, Vol 20, p9
- ISSN
1675-8544
- Publication type
Abstract