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- Title
Phospholipase C-mediated calcium signalling is required for fungal development and pathogenicity in Magnaporthe oryzae.
- Authors
HEE-SOOL RHO; JUNHYUN JEON; YONG-HWAN LEE
- Abstract
Calcium signalling has profound implications in the fungal infection of plants and animals, during which a series of physiological and morphological transitions are required. In this article, using a model fungal pathogen, Magnaporthe oryzae, we demonstrate that the regulation of the intracellular calcium concentration ([Ca2+]int) is essential for fungal development and pathogenesis. Imaging of [Ca2+]int showed that infection-specific morphogenesis is highly correlated with the spatiotemporal regulation of calcium flux. Deletion of the fungal phospholipase C gene ( M. oryzae phospholipase C 1, MoPLC1) suppressed calcium flux, resulting in a fungus defective in developmental steps, including appressorium formation and pathogenicity. Surprisingly, the PLC-δ1 gene of mouse was able to functionally substitute for MoPLC1 by restoring the calcium flux, suggesting the evolutionary conservation of the phospholipase C-mediated regulation of calcium flux. Our results reveal that MoPLC1 is a conserved modulator of calcium flux that is essential for the regulation of key steps in fungal development and pathogenesis.
- Subjects
MYCOSES; PHOSPHOLIPASE C; PYRICULARIA grisea; DISEASE resistance of plants; MORPHOGENESIS; DEVELOPMENTAL biology; LABORATORY mice; PHOSPHOLIPASES; BOTANY study &; teaching
- Publication
Molecular Plant Pathology, 2009, Vol 10, Issue 3, p337
- ISSN
1464-6722
- Publication type
Article
- DOI
10.1111/j.1364-3703.2009.00536.x