We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Pooled DNA sequencing in hairy vetch (Vicia villosa Roth) reveals QTL for seed dormancy but not pod dehiscence.
- Authors
Tilhou, Neal; Kucek, Lisa Kissing; Carr, Brandon; Douglas, Joel; Englert, John; Ali, Shahjahan; Raasch, John; Bhamidimarri, Suresh; Mirsky, Steven; Monteros, Maria J.; Hayes, Ryan; Riday, Heathcliffe
- Abstract
Introduction: Hairy vetch (Vicia villosa Roth) is a promising legume cover crop, but its use is limited by high rates of pod dehiscence and seed dormancy. Methods: We used phenotypically contrasting pooled DNA samples (n=24 with 29- 74 individuals per sample) from an ongoing cover crop breeding program across four environments (site-year combinations: Maryland 2020, Maryland 2022, Wisconsin 2021,Wisconsin 2022) to find genetic associations and genomic prediction accuracies for pod dehiscence and seed dormancy. We also combined pooled DNA sample genetic association results with the results of a prior genome-wide association study. Results and discussion: Genomic prediction resulted in positive predictive abilities for both traits between environments and with an independent dataset (0.34-0.50), but reduced predictive ability for DNA pools with divergent seed dormancy in the Maryland environments (0.07-0.15). The pooled DNA samples found six significant (false discovery rate q-value<0.01) quantitative trait loci (QTL) for seed dormancy and four significant QTL for pod dehiscence. Unfortunately, the minor alleles of the pod dehiscence QTL increased the rate of pod dehiscence and are not useful for markerassisted selection. When combined with a prior association study, sixteen seed dormancy QTL and zero pod dehiscence QTL were significant. Combining the association studies did not increase the detection of useful QTL.
- Subjects
MARYLAND; WISCONSIN; SEED dormancy; DNA sequencing; LOCUS (Genetics); GENOME-wide association studies; PLANT breeding
- Publication
Frontiers in Plant Science, 2024, p1
- ISSN
1664-462X
- Publication type
Article
- DOI
10.3389/fpls.2024.1384596