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- Title
Genetic polymorphism in Caulerpa taxifolia (Ulvophyceae) chloroplast DNA revealed by a PCR-based assay of the invasive Mediterranean strain.
- Authors
Famà, P.; Jousson, O.; Zaninetti, L.; Meinesz, A.; Dini, F.; Di Giuseppe, G.; Millar, A. J. K.; Pawlowski, J.
- Abstract
Abstract An invasive, cold-tolerant strain of the tropical green alga Caulerpa taxifolia was introduced recently in the Mediterranean Sea and along the Californian coast. We screened 50 aquarium and open-sea C. taxifolia specimens for the presence/absence of an intron located in the rbc L gene of chloroplast DNA. We also reanalysed a total of 229 sequences of the Internal Transcribed Spacer (ITS) of ribosomal DNA, combining previously published sequences from different studies with 68 new sequences to complement rbc L data. The introduced Mediterranean strain was found to be characterized by the absence of the rbc L intron and by the occurrence of a particular monomorphic ITS type. A PCR assay based on rbc L gene was developed to detect new introductions of the invasive strain of C. taxifolia . This rapid and inexpensive test could be useful to assist environment managers in the preservation of coastal marine ecosystems.
- Subjects
CAULERPA taxifolia; GENETIC polymorphisms; DNA
- Publication
Journal of Evolutionary Biology, 2002, Vol 15, Issue 4, p618
- ISSN
1010-061X
- Publication type
Article
- DOI
10.1046/j.1420-9101.2002.00418.x