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- Title
Flavonoid-rich extract of <italic>Chromolaena odorata</italic> modulate circulating GLP-1 in Wistar rats: computational evaluation of TGR5 involvement.
- Authors
Omotuyi, Olaposi Idowu; Nash, Oyekanmi; Inyang, Olumide Kayode; Ogidigo, Joyce; Enejoh, Ojochenemi; Okpalefe, Okiemute; Hamada, Tsuyoshi
- Abstract
<italic>Chromolaena odorata</italic> is a major bio-resource in folkloric treatment of diabetes. In the present study, its anti-diabetic component and underlying mechanism were investigated. A library containing 140 phytocompounds previously characterized from <italic>C. odorata</italic> was generated and docked (Autodock Vina) into homology models of dipeptidyl peptidase (DPP)-4, Takeda-G-protein-receptor-5 (TGR5), glucagon-like peptide 1 (GLP1) receptor, renal sodium dependent glucose transporter (SGLUT)-1/2 and nucleotide-binding oligomerization domain (NOD) proteins 1&2. GLP-1 gene (RT-PCR) modulation and its release (EIA) by <italic>C. odorata</italic> were confirmed in vivo. From the docking result above, TGR5 was identified as a major target for two key C. odorata flavonoids (5,7-dihydroxy-6-4-dimethoxyflavanone and homoesperetin-7-rutinoside); sodium taurocholate and <italic>C. odorata</italic> powder included into the diet of the animals both raised the intestinal GLP-1 expression versus control (<italic>p</italic> < 0.05); When treated with flavonoid-rich extract of <italic>C. odorata</italic> (CoF) or malvidin, circulating GLP-1 increased by 130.7% in malvidin-treated subjects (0 vs. 45 min). CoF treatment also resulted in 128.5 and 275% increase for 10 and 30 mg/kg <italic>b.w.,</italic> respectively. Conclusions: The results of this study support that <italic>C. odorata</italic> flavonoids may modulate the expression of GLP-1 and its release via TGR5. This finding may underscore its anti-diabetic potency.
- Subjects
CHROMOLAENA odorata; FLAVONOIDS; REVERSE transcriptase polymerase chain reaction; G protein coupled receptors; SODIUM compounds
- Publication
3 Biotech, 2018, Vol 8, Issue 2, p0
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-018-1138-x