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- Title
Optimized production and isolation of antibacterial agent from marine Aspergillus flavipes against Vibrio harveyi.
- Authors
Guo, Lei; Wang, Cong
- Abstract
Statistical methodologies, including Plackett-Burman design and Box-Behnken design, were employed to optimize the fermentation conditions for the production of active substances against aquatic pathogen Vibrio harveyi by marine-derived Aspergillus flavipes strain HN4-13. The optimal crucial fermentation values for maximum production of active substances against V. harveyi were obtained as follows: X (peptone) = 0.3%, X (KCl) = 0.25%, and X (inoculum size) = 4.5%. The predicted diameter of inhibitory zone against V. harveyi was 23.39 mm, and the practical value reached 23.71 ± 0.98 mm with a 62.3% increase. Bioassay-guided fractionation resulted in the acquisition of two compounds whose structures were identified as questin ( 1) and emodin ( 2). Questin exhibited the same antibacterial activity against V. harveyi as streptomycin (MIC 31.25 µg/mL). This is the first time to report questin as a potential antibacterial agent against aquatic pathogen V. harveyi.
- Subjects
ASPERGILLUS; VIBRIO harveyi; ANTIBACTERIAL agents; ANTI-infective agents; FERMENTATION; DOSE fractionation; RESPONSE surfaces (Statistics)
- Publication
3 Biotech, 2017, Vol 7, Issue 6, p1
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-017-1015-z