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- Title
ZINC AS PROMOTER OF GROWTH AND BIOCHEMICAL ACTIVITY IN BASIL CULTIVARS UNDER in vitro CONDITIONS.
- Authors
Galego Comar, Caroline; Maria Barbosa, Edinara; Rocha Pereira, Vanusa Souza; Destro de Lima, Julliane; Teodoro Santana, Thiago; Mara Magalhães, Hélida
- Abstract
In vitro cultivation of basil allows the manipulation of the concentration of certain micronutrients, commonly neglected by the micropropagation protocols. It is a plant of great economic importance for the cosmetic and pharmaceutical industry, due to the components present in its essential oil. In view of the above, the objective of this study was to evaluate zinc (Zn) concentrations in the micropropagation of basil, in addition to antioxidant activity and total phenolic compounds. Basil seeds, cultivars Manolo and Grecco Palla were oxygenated for 4 h, passed through asepsis and placed in test tubes with MS medium supplemented with 30 g L-1 sucrose and 6.5 g L-1 agar and pH adjusted to 5.8. The treatments were composed by the addition or not of 25 µM of zinc sulfate (ZnSO4) and arranged in a completely randomized design. The tubes containing the seeds and the culture medium were kept in a growth chamber for 90 days. The cultivar Manolo was more sensitive to the addition of ZnSO4 due to the increase in the number of leaves and in the antioxidant activity, however, the addition of this component in the culture medium did not influence the production of phenolic compounds or the activity of the antioxidant enzymes SOD, CAT and APX.
- Subjects
BASIL; PLANT phenols; ZINC sulfate; CULTIVARS; ZINC; PHENOLS; ESSENTIAL oils
- Publication
Scientia Agraria Paranaensis, 2019, Vol 18, Issue 4, p342
- ISSN
1677-4310
- Publication type
Article
- DOI
10.18188/sap.v18i4.21909