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- Title
Preservation of Sudan Red Staining of Brain after Plastination Process.
- Authors
Vibulchan, Punnarat; Intarapanya, Jantima; Cheunsuang, Ornsiri
- Abstract
Specimen preservation is an essential factor in gross anatomical study of the brain and spinal cord. Forced impregnation of the specimen using polymer, or plastination, is to date one of the best specimen preparation procedures. This study aimed to study the possibility of staining whole brain specimen using Sudan Red III dye prior to plastination. Dog brains were divided into 3 groups each stained with the dye for 1, 2 and 3 months, respectively. The stained specimens were then dissected to reveal the staining of deep structures before undergoing the plastination procedure. After the experiment we found that Sudan Red clearly stained the fiber tracts making it differentiable from the gray matter. However, after the strong acetone dehydration steps during the plastination procedure, most of the dye were thinned down in some specimen whereas in a few it was enough to differentiate the gray from white matter although not as clearly as prior to the procedure. Hence, Sudan Red III may not be a reliable dye of choice when followed by plastination.
- Subjects
COLLECTION &; preservation of biological specimens; PLASTINATION; BRAIN research; SPINAL cord; LABORATORY dogs; STAINS &; staining (Microscopy)
- Publication
Thai Journal of Veterinary Medicine, 2012, Vol 42, Issue 3, p379
- ISSN
0125-6491
- Publication type
Article