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- Title
Biochemical and Structural Characterization of Amy1: An Alpha-Amylase from Cryptococcus flavus Expressed in Saccharomyces cerevisiae.
- Authors
Galdino, Alexsandro Sobreira; Silva, Roberto Nascimento; Lottermann, Muriele Taborda; Álvares, Alice CunhaMorales; de Moraes, LídiaMaria Pepe; Torres, Fernando Araripe Gonçalves; de Freitas, SoniaMaria; Ulhoa, Cirano José
- Abstract
An extracellular alpha-amylase (Amy1) whose gene from Cryptococcus flavus was previously expressed in Saccharomyces cerevisiae was purified to homogeneity (67 kDa) by ion-exchange and molecular exclusion chromatography. The enzyme was activated by NH4+ and inhibited by Cu+2 and Hg+2. Significant biochemical and structural discrepancies between wild-type and recombinant a- amylase with respect to Km values, enzyme specificity, and secondary structure content were found. Far-UV CD spectra analysis at pH 7.0 revealed the high thermal stability of both proteins and the difference in folding pattern of Amy1 compared with wild-type amylase from C. flavus, which reflected in decrease (10-fold) of enzymatic activity of recombinant protein. Despite the differences, the highest activity of Amy1 towards soluble starch, amylopectin, and amylase, in contrast with the lowest activity of Amy1w, points to this protein as being of paramount biotechnological importance with many applications ranging from food industry to the production of biofuels.
- Subjects
ALPHA-amylase; STRUCTURAL analysis (Science); MOLECULAR structure; CRYPTOCOCCUS; SACCHAROMYCES cerevisiae; MOLECULAR biology; BIOTECHNOLOGY
- Publication
Enzyme Research, 2011, p1
- ISSN
2090-0406
- Publication type
Article
- DOI
10.4061/2011/157294