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- Title
Role(s) of Leader protein of Saffold virus.
- Authors
Shimizu, Ai; Himeda, Toshiki; Okuwa, Takako; Muraki, Yasushi; Ohara, Yoshiro
- Abstract
Objective Saffold virus ( SAFV) classified in the species Theilovirus of the genus Cardiovirus is a novel human cardiovirus. The pathogenicity of SAFV in humans still remains unclear. Viruses in the genus Cardiovirus produce Leader protein, which is a small non-structural protein. Evidence that Leader protein of murine cardioviruses is a key factor in their biological activities has been provided. Therefore, the studies on the function of SAFV-L are likely to be important in order to clarify the pathogenicity of SAFV. Methods We investigated the interferon ( IFN) response in the early stage of SAFV infection by reverse transcription polymerase chain reaction and the growth kinetics of the chimera virus, SAFL/ DA. Results The study showed that SAFV-L suppresses the type I IFN response, and that the responsible region of anti- IFN activity of SAFV-L is a zinc-binding motif. Furthermore, the anti-apoptotic activity of SAFV-L was suggested from the growth kinetics analysis of SAFL/ DA. Conclusions The present study suggested that SAFV-L might be a multifunctional protein; for example, the suppression of IFN induction, anti-apoptotic effect and/or productive virus growth.
- Subjects
THEILER'S murine encephalomyelitis virus; PICORNAVIRUSES; INTERFERONS; VIRUS diseases; PROTEINS
- Publication
Clinical & Experimental Neuroimmunology, 2014, Vol 5, Issue 3, p362
- ISSN
1759-1961
- Publication type
Article
- DOI
10.1111/cen3.12109